British brothers Neal and Ken Skupski lost 6-3 6-4 to Finland’s Henri Kontinen and Australia’s John Peers in the second round of the men’s doubles at the Australian Open.

The pair have never gone beyond round two in Melbourne.

They comfortably beat Australians James Duckworth and Jordan Thompson 6-3 6-4 in the first round.

Briton Dom Inglot is in men’s doubles action later on Friday and Harriet Dart plays in the women’s doubles.

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Britain’s Johanna Konta was knocked out of the Australian Open in a second-round match against Garbine Muguruza which finished at 03:12 local time.

Konta, 27, lost 6-4 6-7 (3-7) 7-5 in Melbourne in one of the latest finishes in the tournament’s history.

Spaniard Muguruza, 25, nicked a tight final set in front of about 250 remaining fans on Margaret Court Arena.

“I don’t agree with athletes having to physically exert themselves in the early hours,” Konta told BBC Sport.

“I don’t think it is healthy – in fact I think it is quite dangerous.

“In the circumstances I thought we put on a great match and it’s a shame more people couldn’t see it.”

Konta’s defeat means there are no British players left in the singles in Melbourne.

She said they were not given the option to postpone the match until day five.

The latest finish in Australian Open history is a 2008 third-round match between Lleyton Hewitt and Marcos Baghdatis that ended at 04:34.

Two-time Grand Slam champion Muguruza took her first match point to win in two hours and 42 minutes and reach the third round.

“I can’t believe there are people watching us at 3:15am,” the former world number one said.

“We play for you guys watching – otherwise why are we here?

“It was very tough. Johanna played very good, serving incredibly and hitting big shots. I just tried to hang in there.”

Konta pipped in match which neither deserved to lose

The players did not walk out on to Melbourne Park’s second show court until 00:22 local time after the final match of the day was pushed back as a result of Kei Nishikori’s win over Ivo Karlovic and Alexander Zverev’s victory against Jeremy Chardy both taking almost four hours.

When the match between Zverev and Chardy went into a fifth set, tournament organisers were planning to switch the match to court three – but halted that plan with fourth seed Zverev rattling towards victory.

British number one Konta threw the first ball at 00:30 local time in what was an eerie atmosphere in front of a sparse crowd in the 10,000-seat arena.

Despite the enforced wait, both women produced a high-quality match in which they provided reminders of their pedigrees after falling down the rankings in recent times.

The match could have swung either way, with defeat harsh on whoever ended up the loser.

Konta hit a forehand into the net after Muguruza upped the ante to clinch victory with only the second break of the match.

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Britain’s final singles hope bows out

Konta was Britain’s last remaining singles hope at the first Grand Slam of the year, having seen seven of the nation’s players – including Andy Murray and men’s number one Kyle Edmund – lose in the opening three days.

Konta, now ranked 38th in the world, reached the Melbourne semi-finals in 2016 but a pronounced dip in form saw her drop as low as 50th in the world last summer.

Despite the nature of the defeat, she will take encouragement from one of her best performances in recent times.

Konta made a slow start, losing her serve in the opening game and unable to win a point on Muguruza’s first two service games.

But she grew into the contest and matched Muguruza’s power in an entertaining battle.

Konta hit 13 winners in the first set as she attacked, but was unable to convert a break point for 3-4 when she missed a cross-court forehand – one of 13 unforced errors which ultimately proved the difference.

Both missed break points in a tight second set – Konta in the fourth game and Muguruza in the ninth – leading to a tie-beak.

Konta earned a mini-break with a wonderful deep backhand, moved to the brink with an ace down the middle for 6-3, then took the match into a decider with a forehand winner.

Serve continued to dominate in the final set, with Konta winning 31% of receiving points and Muguruza’s 29%, and not a single break point on offer.

A final-set tie-break looked destined to settle the match until Muguruza seized control.

World number one Novak Djokovic reached the Australian Open third round with a straight-set win over Jo-Wilfried Tsonga.

The six-time champion in Melbourne beat the 33-year-old Frenchman 6-3 7-5 6-4.

Tsonga, whose ranking has fallen to 177 after a knee injury restricted him to 12 matches in 2018, showed flashes of his ability but the Serb, 31, was always in control.

Djokovic faces 19-year-old Canadian Denis Shapovalov, the 25th seed, next.

The match against Tsonga was a repeat of the 2008 final in Melbourne, which the Serb won in four sets to secure his first Grand Slam title.

“You could feel the tension on court at the beginning. We felt a lot of emotion and tried to play a great game,” said Djokovic.

“It’s always tough to play Jo. He has struggled with injury and his ranking doesn’t do him justice in my eyes.”

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Eighth seed Kei Nishikori survived a fightback from Ivo Karlovic to win a final-set tie-break and reach the third round of the Australian Open.

The Japanese player took a two-set lead, before 39-year-old Karlovic, who served 59 aces, recovered to force a decider on Margaret Court Arena.

Nishikori held his nerve to edge out the Croat 6-3 7-6 (8-6) 5-7 5-7 7-6 (10-7) in three hours and 48 minutes.

Meanwhile, Milos Raonic beat 2014 champion Stan Wawrinka in four sets.

Raonic will face Pierre-Hugues Herbert in round three after the Frenchman beat 24th seed Chung Hyeon 6-2 1-6 6-2 6-4.

The South Korean, who reached the semi-finals at Melbourne Park last year, is likely to drop out of the world’s top 50 after the tournament.

Seventh seed Dominic Thiem retired injured from his match against wildcard Alexei Popyrin when trailing 7-5 6-4 2-0.

The Austrian, who had shown signs of frustration that boiled over into smashing his racquet, had come through a draining five-setter in his opening match against Benoit Paire and never looked comfortable against 19-year-old Australian Popyrin.

“I felt very bad during the game, obviously,” Thiem said.

“I started to feel like my whole body hurt, generally not feeling well at all, and in the match it got worse. [My] whole body was hurting, I was feeling weak, and there was no sense to continue.”

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Nishikori saved three break points at 4-4 in the final set and was then 6-7 down in the decisive tie-break, as Karlovic stood on the brink of becoming the oldest man to reach the third round since 44-year-old Australian Ken Rosewall in 1978.

“It was a tough match which could have gone both ways. He almost had it for sure,” said Nishikori, a three-time quarter-finalist at Melbourne Park,

“I focused well on the last points, I’m very happy to win. We both played great tennis – of course he served really well.”

He faces Joao Sousa after the Portuguese beat Philipp Kohlschreiber 7-5 4-6 7-6 (7-4) 5-7 6-4.

Canada’s Raonic was forced to come from a set down to see off Swiss three-time Grand Slam champion Wawrinka, who was unseeded for the first time since his debut in 2006 after injuries saw him drop to 59th in the world.

Raonic, the 16th seed, won 6-7 (4-7) 7-6 (8-6) 7-6 (13-11) 7-6 (7-5) in four hours one minute.

“It feels like four hours passed by in about 15 minutes,” he said.

“It was the heat of the battle – the adrenaline takes over and I was fortunate to stay alive in that fourth set.

“I am thankful I was able to play a good match.”

Frenchman Gilles Simon, the 29th seed, was beaten in five sets by Australian wildcard Alex Bolt, who is ranked 159th in the world.

In a match lasting more than four hours, Bolt won 2-6 6-4 4-6 7-6 (10-8) 6-4 to set up a third-round encounter with fourth seed Alexander Zverev or Frenchman Jeremy Chardy.